Efeito da fotobiomodulação infravermelha associada à proteína da matriz do esmalte na formação óssea com osso bovino desproteinizado em ratos

Abstract

Objective: This study evaluated whether the combination of infrared photobiomodulation therapy (IR-PBMT) and enamel matrix derivative (EMD) enhances the repair of areas grafted with deproteinized bovine bone (DBB). Material and methods: Forty-eight rats were used. Surgery was performed bilaterally in the mandible, with both sides allocated to the same experimental group. The animals were evaluated at two experimental periods (30 and 90 days; n = 6). A dome shaped Teflon was placed in the mandibular ramus of each animal, bilaterally, and filled with DBB. The groups were divided according to the type of treatment applied to the grafted area: DBB: no adjunct treatment; EMD: DBB associated with EMD; IR-PBMT: DBB subjected to IR-PBMT; IRPBMT/EMD: DBB associated with EMD + IR-PBMT. The following analyses were performed: (1) micro-computed tomography, to evaluate the volume and microstructure of the grafted area, and (2) histomorphometry, to assess the composition of the repaired tissue. Results: The DBB and IR-PBMT/EMD groups showed greater mineralized tissue volume and smaller trabecular spacing compared to the other groups (p<0.05). The DBB group showed a smaller amount of bone than all other groups at 30 days (p<0.05). The IR-PBMT/EMD group showed a greater amount of bone than the other groups at 90 days (p<0.05). Conclusion: The combined treatment with IRPBMT and EMD enhances repair in areas grafted with DBB.