1. Repositório Institucional - Universidade Federal de Uberlândia
2. Faculdade de Odontologia (FOUFU)
3. TCC - Odontologia

ORCID:	http://orcid.org/0000-0002-5342-3539
Document type:	Trabalho de Conclusão de Curso
Access type:	Acesso Aberto
Title:	Investigating Microarchitectural Characteristics of Woven Bone Exposed to Ionizing Radiation: Comparative Analysis of Two Segmentation Methods and Their Impact on Trabecular Measurements in an Animal Study
Author:	Bites, Clara de Oliveira Barbosa
First Advisor:	Soares, Priscilla Barbosa Ferreira
First coorientator:	Pitorro, Tássio Edno Atanásio
First member of the Committee:	Soares, Priscilla Barbosa Ferreira
Second member of the Committee:	Lima, Lívia Bonjardim
Third member of the Committee:	Henriques, João César Guimarães
Summary:	The study aimed to evaluate bone formation in surgical defects exposed to ionizing radiation (IR) and to compare two image segmentation methods: manual and standardized delimitation of the region-ofinterest (ROI). Bone defects were created in the tibias of Wistar rats using a 2.3 mm trephine. After seven days, the animals were exposed to IR (30 Gy single dose). Two weeks later, the animals were euthanized, and their tibias were scanned using X-ray microtomography. ROI definition was performed by a single operator using two methods: (a) interactive segmentation, where the woven bone was manually outlined within the bone defect region, and (b) a standardized, predefined rectangular ROI. Bone volume fraction (BV/TV), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp), trabecular number (Tb.N), bone surface (BS/BV), and fractal dimension (FD) were assessed for both segmentation methods. Significant differences were observed for BV/TV (p<0.001), Tb.N (p<0.001), and Tb.Sp (p<0.001), but not for Tb.Th (p=0.71), BS/BV (p=0.79), and FD (p=0.35). The manual method was approximately 3.5 times more timeconsuming than the predefined ROI method (446.2±49.5 vs 131.2±38.8 s, p<0.001). None of the evaluated parameters showed significant differences when the IR was considered. Within the limitations of this study, the finding indicate that IR does not interfere with the bone repair process when delivered postsurgery. While manual segmentation is more time consuming, it retrieves greater bone volume and reveals differences in trabecular structure, showing more trabeculae number and less trabeculae separation in irradiated bone.
Keywords:	Bone Fractals X-Ray Microtomography
Area (s) of CNPq:	CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
Language:	eng
Country:	Brasil
Publisher:	Universidade Federal de Uberlândia
Quote:	BITES, Clara de Oliveira Barbosa. Investigating Microarchitectural Characteristics of Woven Bone Exposed to Ionizing Radiation: Comparative Analysis of Two Segmentation Methods and Their Impact on Trabecular Measurements in an Animal Study. 2025. 18 f. Trabalho de Conclusão de Curso (Graduação em Odontologia) – Universidade Federal de Uberlândia, Uberlândia, 2025.
URI:	https://repositorio.ufu.br/handle/123456789/45316
Date of defense:	31-Mar-2025
Appears in Collections:	TCC - Odontologia

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