1. Repositório Institucional - Universidade Federal de Uberlândia
2. Instituto de Ciências Biomédicas (ICBIM)
3. TCC - Ciências Biomédicas

Tipo do documento:	Trabalho de Conclusão de Curso
Tipo de acesso:	Acesso Embargado
Término do embargo:	2025-12-09
Título:	Antimicrobial, antibiofilm, synergistic activities, toxicity in vitro and in vivo of copper(II) complexes with thiosemicarbazone against oral pathogens
Título(s) alternativo(s):	Atividades antimicrobiana, antibiofilme, sinérgica, toxicidade in vitro e in vivo de complexos de cobre(II) com tiossemicarbazona contra patógenos bucais
Autor(es):	Souza, Sara Lemes de
Primeiro orientador:	Martins, Carlos Henrique Gomes
Primeiro membro da banca:	Silva, Helisângela
Segundo membro da banca:	Guerra, Wendell
Resumo:	Caries represents a prevalent public health concern worldwide. This oral condition involves intricate interactions such as biofilm formation and contributes to the development of additional pathological conditions. In the pursuit of antimicrobial agents, metallic compounds have garnered significant attention because of their large biological activity, including antibacterial features. In this scenario, Copper(II) with thiosemicarbazone Shiff base encoded [CuCl(atc-Me)] (1) and [{Cu(μ-atc-Me)}2μ-SO4] (2) have shown promising antimicrobial activity as showed by previously result. To evaluate their anticariogenic potential, assays such as Minimum Inhibitory Concentration (MIC), Minimal Bactericidal Concentration (MBC), Minimum Inhibitory Concentration of Biofilm 50% (MICB50) and synergistic activity with chlorhexidine and for the first time the toxicity of thiosemicarbazone was evaluate in Caenorhabditis elegans. The lowest value of MIC was 0,78 μg/mL for Streptococcus mutans, S. mitis, S. sanguinis and S. sobrinus and MBC presented values of bactericidal activity. The synergic activity with chlorhexidine demonstrated a synergic activity for S. mutans and Enterococcus faecalis. Cytotoxic evaluation in human fibroblast cultures revealed that complexes 1 and 2 showed IC50 equivalent to 2.9 µg/mL and 4.9 µg/mL respectively, being higher than the most promising antibacterial concentrations. The toxicity in C. elegans reveals a lethal concentration that kills 50% of larvae 3x higher than MIC for complexes 1 and 2. The binding energy analyzed by molecular docking for compounds 1 and 2 revels values in a range of -4.78 to −7.79 kcal/mol, respectively. This work points towards an alternative for the cariogenic treatment, especially because of the great activity against biofilm and toxicity above what is necessary.
Palavras-chave:	Copper complexes Thiosemicarbazone Oral pathogens Antibiofilm activity Cytotoxicity C. elegans Molecular docking
Área(s) do CNPq:	CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA APLICADA
Idioma:	eng
País:	Brasil
Editora:	Universidade Federal de Uberlândia
Referência:	SOUZA, Sara Lemes de. Antimicrobial, antibiofilm, synergistic activities, toxicity in vitro and in vivo of copper(II) complexes with thiosemicarbazone against oral pathogens. 2023. 31 f . Trabalho de Conclusão de Curso (Graduação em Biomedicina) - Universidade Federal de Uberlândia, Uberlândia, 2023
URI:	https://repositorio.ufu.br/handle/123456789/39886
Data de defesa:	9-Dez-2023
Aparece nas coleções:	TCC - Ciências Biomédicas

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