1. Repositório Institucional - Universidade Federal de Uberlândia
2. Instituto de Ciências Biomédicas (ICBIM)
3. TCC - Ciências Biomédicas

Document type:	Trabalho de Conclusão de Curso
Access type:	Acesso Embargado
Embargo Date:	2025-12-09
Title:	Antimicrobial, antibiofilm, synergistic activities, toxicity in vitro and in vivo of copper(II) complexes with thiosemicarbazone against oral pathogens
Alternate title (s):	Atividades antimicrobiana, antibiofilme, sinérgica, toxicidade in vitro e in vivo de complexos de cobre(II) com tiossemicarbazona contra patógenos bucais
Author:	Souza, Sara Lemes de
First Advisor:	Martins, Carlos Henrique Gomes
First member of the Committee:	Silva, Helisângela
Second member of the Committee:	Guerra, Wendell
Summary:	Caries represents a prevalent public health concern worldwide. This oral condition involves intricate interactions such as biofilm formation and contributes to the development of additional pathological conditions. In the pursuit of antimicrobial agents, metallic compounds have garnered significant attention because of their large biological activity, including antibacterial features. In this scenario, Copper(II) with thiosemicarbazone Shiff base encoded [CuCl(atc-Me)] (1) and [{Cu(μ-atc-Me)}2μ-SO4] (2) have shown promising antimicrobial activity as showed by previously result. To evaluate their anticariogenic potential, assays such as Minimum Inhibitory Concentration (MIC), Minimal Bactericidal Concentration (MBC), Minimum Inhibitory Concentration of Biofilm 50% (MICB50) and synergistic activity with chlorhexidine and for the first time the toxicity of thiosemicarbazone was evaluate in Caenorhabditis elegans. The lowest value of MIC was 0,78 μg/mL for Streptococcus mutans, S. mitis, S. sanguinis and S. sobrinus and MBC presented values of bactericidal activity. The synergic activity with chlorhexidine demonstrated a synergic activity for S. mutans and Enterococcus faecalis. Cytotoxic evaluation in human fibroblast cultures revealed that complexes 1 and 2 showed IC50 equivalent to 2.9 µg/mL and 4.9 µg/mL respectively, being higher than the most promising antibacterial concentrations. The toxicity in C. elegans reveals a lethal concentration that kills 50% of larvae 3x higher than MIC for complexes 1 and 2. The binding energy analyzed by molecular docking for compounds 1 and 2 revels values in a range of -4.78 to −7.79 kcal/mol, respectively. This work points towards an alternative for the cariogenic treatment, especially because of the great activity against biofilm and toxicity above what is necessary.
Keywords:	Copper complexes Thiosemicarbazone Oral pathogens Antibiofilm activity Cytotoxicity C. elegans Molecular docking
Area (s) of CNPq:	CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA APLICADA
Language:	eng
Country:	Brasil
Publisher:	Universidade Federal de Uberlândia
Quote:	SOUZA, Sara Lemes de. Antimicrobial, antibiofilm, synergistic activities, toxicity in vitro and in vivo of copper(II) complexes with thiosemicarbazone against oral pathogens. 2023. 31 f . Trabalho de Conclusão de Curso (Graduação em Biomedicina) - Universidade Federal de Uberlândia, Uberlândia, 2023
URI:	https://repositorio.ufu.br/handle/123456789/39886
Date of defense:	9-Dec-2023
Appears in Collections:	TCC - Ciências Biomédicas

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