Mutagênese computacional da proteína transportadora de glicina 2, expressa pelo gene SLC6A5 em bovinos da raça Belgian Blue

Abstract

Actually, one of the most prominent breeds of European beef cattle known as Belgian Blue is affected by recessive genetic defects that have resulted in mutations linked to muscle disorders that raise concerns about the animal welfare, health, reproduction, as well as economic losses. For a better understanding of muscle disorders caused by mutation, this study sought to address congenital muscular dystonia (DMC II), a disorder that is little studied and known in animal science, which affects calves in the early hours, interdisciplinary studies were also carried out to understand the effects of genetic mutation in other species. In the current context, genetic studies have become a fundamental pillar for animal selection and genetic improvement, since new tools and methods have been developed to diagnose genes of interest to animal production, in addition to contributing to dynamic studies for a better understanding of the genome and its functions and influence on organisms. This study extended over methodologies from large areas of genetics, such as molecular genetics and mutagenesis, applied to animal genetic improvement to evaluate the effect of mutation by inbreeding in bovine herds. For that, using Molecular Modeling techniques, two mutations were performed in the glycine transporter protein 2 (GLYT2) and subjected to Molecular Dynamics with a simulation time of 20ns and were verified significative changes in the protein conformation, fluctuation, stability and flexibility, resulting in an enzymatic functionality.