Atividade biológica in vitro e in silico de complexos de metais de transição puros e nanoformulados

Abstract

Cancer is a disease that is increasingly affecting a greater number of peoples around the world, being one of the main health problems faced by governments in most countries, causing expenses that have increased greatly in recent decades. Current treatments face difficulties such as cellular resistance and serious side effects because they are unspecific. The emergence of drug delivery nanosystems can significantly improve tissue permeability, prolong drug circulation time and reduce side effects. The reduction of unwanted effects relies on the selectivity mechanism that some transition metal molecules and their ligands present. In the search for therapeutic alternatives that are more selective and less toxic, the present study presents a formulation with a copper(II) complex, already known for its cytotoxic activity, based on lipid nanocarriers and its characterization regarding stability and biological activity. . This formulated complex was stable, indicating a good association between the lipid carrier nanocarrier and the complex. And yet biological activity, as it has a good Selectivity Index and ability to break single and double strands of DNA, by possible interaction with the minor groove, as shown by molecular Docking. It also exhibited the ability to cause dysfunction in the autophagic pathway. With the ability to have antitumor action, the NLC/CBP01 nanoformulation is an excellent candidate for antitumor drug.