Pesquisa da produção de Metalo-β-Lactamases em Pseudomonas aeruginosa pelo teste de ensaio microbiológico EDTA-IMIPENEM (EIM)

Abstract

Health Care-Related Infections are a major public health problem, becoming a challenge due to the direct relationship with antimicrobial resistance. The most frequent infections are those of the respiratory tract, bloodstream and urinary tract and are usually caused by Gram negative pathogens. Among these, it is worth mentioning Pseudomonas aeruginosa, which is an opportunistic bacterium and in most cases, its strains are resistant to antimicrobials, such as carbapenems, increasing the mortality rate. Thus, the present study aimed to investigate the production of metallo-β-lactamases in Pseudomonas aeruginosa and its relationship with the profile of antimicrobial resistance in clinical isolates from patients admitted to the Hospital and Maternity Hospital of Uberlândia. For this, 58 samples resistant to carbapenems were analyzed, which were submitted to phenotypic tests of microbiological assay with imipenem, zinc sulfate and EDTA. Most were from serious infections such as pneumonia and bloodstream infections, in patients over 60 years of age. In addition, most samples were also shown to be resistant to fluoroquinolones, that is, most strains were extensively resistant, with no distinction between the hospital environment in which they were collected. The results also showed that the phenotypic tests were not more efficient when compared to each other. The study pointed out that P. aeruginosa is endemic throughout the hospital and not only in the ICU, with the majority of multisensitive strains being found there, while pan-resistant samples were present in other hospital settings.