Análise genômica comparativa da Xanthomonas arboricola pv. juglandis 417 e estudo in silico do gene copb de resistência ao cobre

Abstract

Xanthomonas arboricola pv. juglandis is an organism with present resistance to the metal Copper and the cause of bacterial blight, pythopatogen that affects Walnut trees worldwide. Via in silico studies of the species, molecular level analyses of hypotheses about the cop operon and the copB resistance gene and in addition, genomic comparisons between the bacterial species and two other phylogenetically close species were performed. Using genomic comparison softwares such as EDGAR 3.0 and MAUVE, it was possible to identify the organisms phylogenetically closest to the reference organism of this study: Xanthomonas campestris pv. campestris, with 253 compatible sequences and Xanthomonas hortorum pv. carotae with 148 compatible sequences. Based on the result of similarity identification in alignments, generated by BLAST2GO software, 5 genes related to copper resistance were expressed: copA, copB, copC and copD, where the copB gene was expressed twice. Along with the mentioned genes, other genes related to resistance functionality were also identified: amine oxidase, copper ABC transporter ATPase, copper homeostasis protein cutC, multicopper oxidase (MCO), copG family transcriptional regulator and copper chaperone PCu(A)C. The copB 119 prediction of biological process indicates response to copper ion, a parity with copB 3389 regarding the biological process. Similarity between copB genes: 58,9%, Identitity between copB genes: 47,0%, and RMSD value of copB genes structural alignments is 3.22.