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https://repositorio.ufu.br/handle/123456789/32898| Tipo do documento: | Trabalho de Conclusão de Curso |
| Tipo de acesso: | Acesso Aberto |
| Título: | Uma avaliação laboratorial da viabilidade celular, radiopacidade e descoloração dentária induzida por materiais endodônticos regenerativos |
| Título(s) alternativo(s): | A laboratory evaluation of cell viability, radiopacity and tooth discoloration induced by regenerative endodontic materials |
| Autor(es): | Magalhães, Thamara Eduarda Alves |
| Primeiro orientador: | Moura, Camilla Christian Gomes |
| Primeiro coorientador: | Oliveira, Lilian Vieira |
| Primeiro membro da banca: | Oliveira, Maria |
| Segundo membro da banca: | Soares, Priscilla |
| Resumo: | Aim: To analyse the cytotoxicity, colour change and radiopacity of MTA Flow (MTA), UltraCal XS (UC) and Bio-C Temp (BT). Methodology: Human dental pulp cells (hDPCs) stimulated with lipopolysaccharide (LPS) were placed in contact with several dilutions of culture media previously exposed to the experimental materials and tested for cell viability using MTT. Bovine teeth were prepared to simulate an open apex and to mimic extensive crown fracture. The roots were filled with a mixture of agar and blood, and the materials placed over this mixture. The control group consisted of teeth filled only with agar and blood. Colour assessment analyses were performed before and immediately after material insertion and repeated at 30, 45 and 60 days using a spectrophotometer. The total colour change (DEab, DE00 and whiteness index (WI)) was calculated based on the CIELAB colour space. Digital radiographs were acquired for radiopacity analysis. Cell viability was analysed by one-way ANOVA, whilst differences in colour parameters (DEab, DE00 and WI) were assessed by two-way repeated measures ANOVA (α = 0.05). Tukey’s test was used to compare the experimental groups, and Dunnett’s test was used to compare the experimental groups with the control group. Results: MTA, UC and BT had similar cell viability to that of the control group (DMEM) (P > 0.05), except for the BT group at the 1 : 1 and 1 : 2 dilutions, which had significantly lower viability (P < 0.001). All materials were associated with discoloration values greater than what is considered to be the acceptable threshold, and BT resulted in less or similar tooth colour change than MTA and UC, respectively. Decreasing radiopacity over time was observed only in the MTA group (P = 0.007). Lower values of radiopacity were found in the BT group compared with the UC and MTA groups (P < 0.001). Conclusions: The new bioceramic material (BT) had acceptable cell viability, similar to that of MTA and UC at the highest dilutions, and BT resulted in less tooth colour change than MTA and UC. Despite its lower radiopacity, BT was identified radiographically. |
| Palavras-chave: | Citotoxicidade Pulpotomia Radiopacidade Potencial de manchamento Descoloração dentária |
| Área(s) do CNPq: | CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
| Idioma: | eng |
| País: | Brasil |
| Editora: | Universidade Federal de Uberlândia |
| Referência: | MAGALHÃES, Thamara Eduarda Alves. Uma avaliação laboratorial da viabilidade celular, radiopacidade e descoloração dentária induzida por materiais endodônticos regenerativos. 2021. Trabalho de Conclusão de Curso (Graduação em Odontologia) - Universidade Federal de Uberlândia, Uberlândia, 2021. |
| URI: | https://repositorio.ufu.br/handle/123456789/32898 |
| Data de defesa: | 5-Out-2021 |
| Aparece nas coleções: | TCC - Odontologia |
Arquivos associados a este item:
| Arquivo | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| AvaliaçãoLaboratorialViabilidade.pdf | 659.99 kB | Adobe PDF |  Visualizar/Abrir |
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