1. Repositório Institucional - Universidade Federal de Uberlândia
2. Faculdade de Odontologia (FOUFU)
3. TCC - Odontologia

Document type:	Trabalho de Conclusão de Curso
Access type:	Acesso Aberto
Title:	Uma avaliação laboratorial da viabilidade celular, radiopacidade e descoloração dentária induzida por materiais endodônticos regenerativos
Alternate title (s):	A laboratory evaluation of cell viability, radiopacity and tooth discoloration induced by regenerative endodontic materials
Author:	Magalhães, Thamara Eduarda Alves
First Advisor:	Moura, Camilla Christian Gomes
First coorientator:	Oliveira, Lilian Vieira
First member of the Committee:	Oliveira, Maria
Second member of the Committee:	Soares, Priscilla
Summary:	Aim: To analyse the cytotoxicity, colour change and radiopacity of MTA Flow (MTA), UltraCal XS (UC) and Bio-C Temp (BT). Methodology: Human dental pulp cells (hDPCs) stimulated with lipopolysaccharide (LPS) were placed in contact with several dilutions of culture media previously exposed to the experimental materials and tested for cell viability using MTT. Bovine teeth were prepared to simulate an open apex and to mimic extensive crown fracture. The roots were filled with a mixture of agar and blood, and the materials placed over this mixture. The control group consisted of teeth filled only with agar and blood. Colour assessment analyses were performed before and immediately after material insertion and repeated at 30, 45 and 60 days using a spectrophotometer. The total colour change (DEab, DE00 and whiteness index (WI)) was calculated based on the CIELAB colour space. Digital radiographs were acquired for radiopacity analysis. Cell viability was analysed by one-way ANOVA, whilst differences in colour parameters (DEab, DE00 and WI) were assessed by two-way repeated measures ANOVA (α = 0.05). Tukey’s test was used to compare the experimental groups, and Dunnett’s test was used to compare the experimental groups with the control group. Results: MTA, UC and BT had similar cell viability to that of the control group (DMEM) (P > 0.05), except for the BT group at the 1 : 1 and 1 : 2 dilutions, which had significantly lower viability (P < 0.001). All materials were associated with discoloration values greater than what is considered to be the acceptable threshold, and BT resulted in less or similar tooth colour change than MTA and UC, respectively. Decreasing radiopacity over time was observed only in the MTA group (P = 0.007). Lower values of radiopacity were found in the BT group compared with the UC and MTA groups (P < 0.001). Conclusions: The new bioceramic material (BT) had acceptable cell viability, similar to that of MTA and UC at the highest dilutions, and BT resulted in less tooth colour change than MTA and UC. Despite its lower radiopacity, BT was identified radiographically.
Keywords:	Citotoxicidade Pulpotomia Radiopacidade Potencial de manchamento Descoloração dentária
Area (s) of CNPq:	CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
Language:	eng
Country:	Brasil
Publisher:	Universidade Federal de Uberlândia
Quote:	MAGALHÃES, Thamara Eduarda Alves. Uma avaliação laboratorial da viabilidade celular, radiopacidade e descoloração dentária induzida por materiais endodônticos regenerativos. 2021. Trabalho de Conclusão de Curso (Graduação em Odontologia) - Universidade Federal de Uberlândia, Uberlândia, 2021.
URI:	https://repositorio.ufu.br/handle/123456789/32898
Date of defense:	5-Oct-2021
Appears in Collections:	TCC - Odontologia

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