Please use this identifier to cite or link to this item: https://repositorio.ufu.br/handle/123456789/32898
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dc.creatorMagalhães, Thamara Eduarda Alves-
dc.date.accessioned2021-10-19T14:46:23Z-
dc.date.available2021-10-19T14:46:23Z-
dc.date.issued2021-10-05-
dc.identifier.citationMAGALHÃES, Thamara Eduarda Alves. Uma avaliação laboratorial da viabilidade celular, radiopacidade e descoloração dentária induzida por materiais endodônticos regenerativos. 2021. Trabalho de Conclusão de Curso (Graduação em Odontologia) - Universidade Federal de Uberlândia, Uberlândia, 2021.pt_BR
dc.identifier.urihttps://repositorio.ufu.br/handle/123456789/32898-
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Uberlândiapt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectCitotoxicidadept_BR
dc.subjectPulpotomiapt_BR
dc.subjectRadiopacidadept_BR
dc.subjectPotencial de manchamentopt_BR
dc.subjectDescoloração dentáriapt_BR
dc.titleUma avaliação laboratorial da viabilidade celular, radiopacidade e descoloração dentária induzida por materiais endodônticos regenerativospt_BR
dc.title.alternativeA laboratory evaluation of cell viability, radiopacity and tooth discoloration induced by regenerative endodontic materialspt_BR
dc.typeTrabalho de Conclusão de Cursopt_BR
dc.contributor.advisor-co1Oliveira, Lilian Vieira-
dc.contributor.advisor1Moura, Camilla Christian Gomes-
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/8078512804636882pt_BR
dc.contributor.referee1Oliveira, Maria-
dc.contributor.referee1Latteshttp://lattes.cnpq.br/4375188137877128pt_BR
dc.contributor.referee2Soares, Priscilla-
dc.contributor.referee2Latteshttp://lattes.cnpq.br/5032809676969673pt_BR
dc.description.degreenameTrabalho de Conclusão de Curso (Graduação)pt_BR
dc.description.resumoAim: To analyse the cytotoxicity, colour change and radiopacity of MTA Flow (MTA), UltraCal XS (UC) and Bio-C Temp (BT). Methodology: Human dental pulp cells (hDPCs) stimulated with lipopolysaccharide (LPS) were placed in contact with several dilutions of culture media previously exposed to the experimental materials and tested for cell viability using MTT. Bovine teeth were prepared to simulate an open apex and to mimic extensive crown fracture. The roots were filled with a mixture of agar and blood, and the materials placed over this mixture. The control group consisted of teeth filled only with agar and blood. Colour assessment analyses were performed before and immediately after material insertion and repeated at 30, 45 and 60 days using a spectrophotometer. The total colour change (DEab, DE00 and whiteness index (WI)) was calculated based on the CIELAB colour space. Digital radiographs were acquired for radiopacity analysis. Cell viability was analysed by one-way ANOVA, whilst differences in colour parameters (DEab, DE00 and WI) were assessed by two-way repeated measures ANOVA (α = 0.05). Tukey’s test was used to compare the experimental groups, and Dunnett’s test was used to compare the experimental groups with the control group. Results: MTA, UC and BT had similar cell viability to that of the control group (DMEM) (P > 0.05), except for the BT group at the 1 : 1 and 1 : 2 dilutions, which had significantly lower viability (P < 0.001). All materials were associated with discoloration values greater than what is considered to be the acceptable threshold, and BT resulted in less or similar tooth colour change than MTA and UC, respectively. Decreasing radiopacity over time was observed only in the MTA group (P = 0.007). Lower values of radiopacity were found in the BT group compared with the UC and MTA groups (P < 0.001). Conclusions: The new bioceramic material (BT) had acceptable cell viability, similar to that of MTA and UC at the highest dilutions, and BT resulted in less tooth colour change than MTA and UC. Despite its lower radiopacity, BT was identified radiographically.pt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.courseOdontologiapt_BR
dc.sizeorduration22pt_BR
dc.subject.cnpqCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIApt_BR
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