Efeito do uso do resveratrol associado à nanopartículas de silíca durante a maturação in vitro de ovócitos bovinos

Abstract

During the in vitro embryo production, the oocyte maturation may be influenced by oxidative stress. This way, it is important to know the means of controlling the production of oxygen reactive species (ROs). The aim of this study was to evaluate the use of resveratrol associated to nanoparticles of silica during the in vitro maturation of bovine oocytes. We collected bovine ovaries in regional slaughterhouses and the oocytes were distributed during the maturation process in the following treatments: control group, resveratrol 0.5 μM, resveratrol 1 μM, resveratrol connected to nanoparticles 0.5 μM, and resveratrol connected to nanoparticles 1 μM. We evaluated the cleavage rate and blastocysts production, ROs production, oocyte nuclear maturation, and cellular apoptosis rate. To measure the production rate, we used 789 oocytes and the Ros and nuclear maturation analysis were made using probes 2',7'- dichlorofluorescin diacetate (DCFDA) and 4',6'-diamidino-2-phenylindole (DAPI), respectively, with 130 and 95 oocytes. The cellular apoptosis rate was done in 117 embryos through propidium iodide. The statistical analysis was made using the Sigma Plot program version 11, and the data were considered significant with P< 0.05 (bilateral). The use of diluted resveratrol at 1µM decreased the cleavage rate of the blastocysts, and there was no difference between the treatments at ROs production, oocyte maturation and cellular apoptosis. The resveratrol shows better results when associated to silica nanoparticles at the cleavage rate and blastocysts related to its diluted form at 1μM concentration, which may be associated to a decrease of the toxicity of the antioxidant because of the controlled release when it is associated to these nanomaterials.