1. Repositório Institucional - Universidade Federal de Uberlândia
2. Instituto de Biotecnologia (IBTEC)
3. TCC - Biotecnologia (Patos de Minas)

ORCID:	http://orcid.org/0000-0002-6628-7185
Tipo do documento:	Trabalho de Conclusão de Curso
Tipo de acesso:	Acesso Aberto
Título:	Genome-wide identification, characterization and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata
Título(s) alternativo(s):	Identificação em todo o genoma, caracterização e perfil de expressão dos genes de ubiquitina-proteassoma em Biomphalaria glabrata
Autor(es):	Duarte, Bruna Custódio Dias
Primeiro orientador:	Gomes, Matheus de Souza
Primeiro membro da banca:	Barbosa, Aulus Estevão Anjos de Deus
Segundo membro da banca:	Freitas, Guilherme Ramos Oliveira e
Resumo:	Abstract BACKGROUND: Biomphalaria glabrata is the major species used for the study of schistosomiasis-related parasite-host relationships, and understanding its gene regulation may aid in this endeavor. The ubiquitin-proteasome system (UPS) performs post-translational regulation in order to maintain cellular protein homeostasis and is related to several mechanisms, including immune responses. OBJECTIVE: The aims of this work were to identify and characterize the putative genes and proteins involved in UPS using bioinformatic tools and also their expression on different tissues of B. glabrata. METHODS: The putative genes and proteins of UPS in B. glabrata were predicted using BLASTp and as queries reference proteins from model organism. We characterized these putative proteins using PFAM and CDD software describing the conserved domains and active sites. The phylogenetic analysis was performed using ClustalX2 and MEGA5.2. Expression evaluation was performed from 12 snail tissue using RPKM. FINDINGS: 119 sequences involved in the UPS in B. glabrata were identified, which 86 have been related to the ubiquitination pathway and 33 to proteasome. In addition, the conserved domains found were associated with the ubiquitin family, UQ_con, HECT, U-box and proteasome. The main active sites were lysine and cysteine residues. Lysines are responsible and the starting point for the formation of polyubiquitin chains, while the cysteine residues of the enzymes are responsible for binding to ubiquitin. The phylogenetic analysis showed an organised distribution between the organisms and the clades of the sequences, corresponding to the tree of life of the animals, for all groups of sequences analysed. The ubiquitin sequence was the only one with a high expression profile found in all libraries, inferring its wide range of performance. MAIN CONCLUSIONS: Our results evidenced the presence, conservation and expression profile of the UPS in this mollusk, providing basis and new knowledge to other studies involving this system. Due to the importance of UPS and B. glabrata, this work may influence the search for new methodologies for the control of schistosomiasis.
Palavras-chave:	Snail Caramujo UPS Signaling pathway Via de sinalização Bioinformatics Bioinformática Schistosomiasis Esquistossomose
Área(s) do CNPq:	CNPQ::CIENCIAS BIOLOGICAS::PARASITOLOGIA
Idioma:	por
País:	Brasil
Editora:	Universidade Federal de Uberlândia
Referência:	DUARTE, Bruna Custódio Dias. Genome-wide identification, characterization and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata. 2019. 46 f. Trabalho de Conclusão de Curso (Graduação em Biotecnologia) - Universidade Federal de Uberlândia, Patos de Minas, 2019.
URI:	https://repositorio.ufu.br/handle/123456789/27815
Data de defesa:	11-Dez-2019
Aparece nas coleções:	TCC - Biotecnologia (Patos de Minas)

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