Please use this identifier to cite or link to this item:
https://repositorio.ufu.br/handle/123456789/27815
ORCID: | http://orcid.org/0000-0002-6628-7185 |
Document type: | Trabalho de Conclusão de Curso |
Access type: | Acesso Aberto |
Title: | Genome-wide identification, characterization and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata |
Alternate title (s): | Identificação em todo o genoma, caracterização e perfil de expressão dos genes de ubiquitina-proteassoma em Biomphalaria glabrata |
Author: | Duarte, Bruna Custódio Dias |
First Advisor: | Gomes, Matheus de Souza |
First member of the Committee: | Barbosa, Aulus Estevão Anjos de Deus |
Second member of the Committee: | Freitas, Guilherme Ramos Oliveira e |
Summary: | Abstract BACKGROUND: Biomphalaria glabrata is the major species used for the study of schistosomiasis-related parasite-host relationships, and understanding its gene regulation may aid in this endeavor. The ubiquitin-proteasome system (UPS) performs post-translational regulation in order to maintain cellular protein homeostasis and is related to several mechanisms, including immune responses. OBJECTIVE: The aims of this work were to identify and characterize the putative genes and proteins involved in UPS using bioinformatic tools and also their expression on different tissues of B. glabrata. METHODS: The putative genes and proteins of UPS in B. glabrata were predicted using BLASTp and as queries reference proteins from model organism. We characterized these putative proteins using PFAM and CDD software describing the conserved domains and active sites. The phylogenetic analysis was performed using ClustalX2 and MEGA5.2. Expression evaluation was performed from 12 snail tissue using RPKM. FINDINGS: 119 sequences involved in the UPS in B. glabrata were identified, which 86 have been related to the ubiquitination pathway and 33 to proteasome. In addition, the conserved domains found were associated with the ubiquitin family, UQ_con, HECT, U-box and proteasome. The main active sites were lysine and cysteine residues. Lysines are responsible and the starting point for the formation of polyubiquitin chains, while the cysteine residues of the enzymes are responsible for binding to ubiquitin. The phylogenetic analysis showed an organised distribution between the organisms and the clades of the sequences, corresponding to the tree of life of the animals, for all groups of sequences analysed. The ubiquitin sequence was the only one with a high expression profile found in all libraries, inferring its wide range of performance. MAIN CONCLUSIONS: Our results evidenced the presence, conservation and expression profile of the UPS in this mollusk, providing basis and new knowledge to other studies involving this system. Due to the importance of UPS and B. glabrata, this work may influence the search for new methodologies for the control of schistosomiasis. |
Keywords: | Snail Caramujo UPS Signaling pathway Via de sinalização Bioinformatics Bioinformática Schistosomiasis Esquistossomose |
Area (s) of CNPq: | CNPQ::CIENCIAS BIOLOGICAS::PARASITOLOGIA |
Language: | por |
Country: | Brasil |
Publisher: | Universidade Federal de Uberlândia |
Quote: | DUARTE, Bruna Custódio Dias. Genome-wide identification, characterization and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata. 2019. 46 f. Trabalho de Conclusão de Curso (Graduação em Biotecnologia) - Universidade Federal de Uberlândia, Patos de Minas, 2019. |
URI: | https://repositorio.ufu.br/handle/123456789/27815 |
Date of defense: | 11-Dec-2019 |
Appears in Collections: | TCC - Biotecnologia (Patos de Minas) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
GenomeWideIdentification.pdf | TCC | 1.62 MB | Adobe PDF | View/Open |
SupplementaryMaterial.pdf | TCC | 3.83 MB | Adobe PDF | View/Open |
Figures.pdf | TCC | 1.6 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License