Genome-wide identification, characterization and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata

Duarte, Bruna Custódio Dias

Please use this identifier to cite or link to this item: https://repositorio.ufu.br/handle/123456789/27815

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DC Field	Value	Language
dc.creator	Duarte, Bruna Custódio Dias	-
dc.date.accessioned	2019-12-18T19:12:34Z	-
dc.date.available	2019-12-18T19:12:34Z	-
dc.date.issued	2019-12-11	-
dc.identifier.citation	DUARTE, Bruna Custódio Dias. Genome-wide identification, characterization and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata. 2019. 46 f. Trabalho de Conclusão de Curso (Graduação em Biotecnologia) - Universidade Federal de Uberlândia, Patos de Minas, 2019.	pt_BR
dc.identifier.uri	https://repositorio.ufu.br/handle/123456789/27815	-
dc.language	por	pt_BR
dc.publisher	Universidade Federal de Uberlândia	pt_BR
dc.rights	Acesso Aberto	pt_BR
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.subject	Snail	pt_BR
dc.subject	Caramujo	pt_BR
dc.subject	UPS	pt_BR
dc.subject	Signaling pathway	pt_BR
dc.subject	Via de sinalização	pt_BR
dc.subject	Bioinformatics	pt_BR
dc.subject	Bioinformática	pt_BR
dc.subject	Schistosomiasis	pt_BR
dc.subject	Esquistossomose	pt_BR
dc.title	Genome-wide identification, characterization and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata	pt_BR
dc.title.alternative	Identificação em todo o genoma, caracterização e perfil de expressão dos genes de ubiquitina-proteassoma em Biomphalaria glabrata	pt_BR
dc.type	Trabalho de Conclusão de Curso	pt_BR
dc.contributor.advisor1	Gomes, Matheus de Souza	-
dc.contributor.advisor1Lattes	http://lattes.cnpq.br/8674610062329213	pt_BR
dc.contributor.referee1	Barbosa, Aulus Estevão Anjos de Deus	-
dc.contributor.referee1Lattes	http://lattes.cnpq.br/5331406701784638	pt_BR
dc.contributor.referee2	Freitas, Guilherme Ramos Oliveira e	-
dc.contributor.referee2Lattes	http://lattes.cnpq.br/4014066906990228	pt_BR
dc.creator.Lattes	http://lattes.cnpq.br/3113248545028163	pt_BR
dc.description.degreename	Trabalho de Conclusão de Curso (Graduação)	pt_BR
dc.description.resumo	Abstract BACKGROUND: Biomphalaria glabrata is the major species used for the study of schistosomiasis-related parasite-host relationships, and understanding its gene regulation may aid in this endeavor. The ubiquitin-proteasome system (UPS) performs post-translational regulation in order to maintain cellular protein homeostasis and is related to several mechanisms, including immune responses. OBJECTIVE: The aims of this work were to identify and characterize the putative genes and proteins involved in UPS using bioinformatic tools and also their expression on different tissues of B. glabrata. METHODS: The putative genes and proteins of UPS in B. glabrata were predicted using BLASTp and as queries reference proteins from model organism. We characterized these putative proteins using PFAM and CDD software describing the conserved domains and active sites. The phylogenetic analysis was performed using ClustalX2 and MEGA5.2. Expression evaluation was performed from 12 snail tissue using RPKM. FINDINGS: 119 sequences involved in the UPS in B. glabrata were identified, which 86 have been related to the ubiquitination pathway and 33 to proteasome. In addition, the conserved domains found were associated with the ubiquitin family, UQ_con, HECT, U-box and proteasome. The main active sites were lysine and cysteine residues. Lysines are responsible and the starting point for the formation of polyubiquitin chains, while the cysteine residues of the enzymes are responsible for binding to ubiquitin. The phylogenetic analysis showed an organised distribution between the organisms and the clades of the sequences, corresponding to the tree of life of the animals, for all groups of sequences analysed. The ubiquitin sequence was the only one with a high expression profile found in all libraries, inferring its wide range of performance. MAIN CONCLUSIONS: Our results evidenced the presence, conservation and expression profile of the UPS in this mollusk, providing basis and new knowledge to other studies involving this system. Due to the importance of UPS and B. glabrata, this work may influence the search for new methodologies for the control of schistosomiasis.	pt_BR
dc.publisher.country	Brasil	pt_BR
dc.publisher.course	Biotecnologia	pt_BR
dc.sizeorduration	46	pt_BR
dc.subject.cnpq	CNPQ::CIENCIAS BIOLOGICAS::PARASITOLOGIA	pt_BR
dc.orcid.putcode	66268376	-
Appears in Collections:	TCC - Biotecnologia (Patos de Minas)