Determinação da fibra em detergente neutro em alimentos volumosos utilizando autoclave com diferentes tempos e temperaturas

Abstract

Several alternative methods to the original method of neutral detergent diber (NDF) analysis were developed using materials and equipment different from the conventional method, such as the use of filter bags instead of filter crucibles and the use of autoclaves instead of the fiber digester. The objective of this work was to evaluate the NDF analysis in three different roughage by autoclave method, using a non-woven fabric (100 g/m2) in the different times and temperature of the operating (105°C for 60 minutes, 110°C for 40 minutes e 120°C for 40 minutes). The experiment design was completely randomized in factorial scheme (3 x3) with three execution times and temperature and three roughages, with 5 repetitions each. The results were submitted to analysis of variance and the averages compared using the Tukey test at 5% probability, using the statistical program R 3.4.2. The NDF content did not differ (p> 0.05) at temperature of 105 °C for 60 minutes and 110 °C for 40 minutes. However, the temperature of 120 °C for 40 minutes generated lower results (p<0.05) compared to the temperature of 105 °C for 60 minutes, but did not differ (p>0.05) from the values obtained with the temperature of 110 °C for 40 minutes. It was concluded that the use of autoclave in the NDF analysis at 105 °C for 60 minutes and 110 °C for 40 minutes yields similar results. However, the temperature of 120 °C for 40 minutes underestimates the NDF content of roughages.