Determinação da fibra em detergente ácido em alimentos volumosos utilizando a autoclave com diferentes tempos e temperaturas de extração

Abstract

The analysis of the fiber content in animal nutrition is of great importance, and several alternative methods to the conventional method arose with the purpose of reducing the labor force, increasing the number of samples analyzed and reducing the execution time of the analysis, among which the use of the autoclave. The objective of the present study was to evaluate the acid detergent fiber content in a sample of three roughage foods: Tifton 85 Grass (Cynodon spp.), Marandu Grass (Urochloa brizantha cv. Marandu) and Xaraés Grass (Urochloa brizantha cv. Xaraés), using the autoclave technique at different temperatures and operating times: 105°C for 60 minutes, 110°C for 40 minutes and 120°C for 40 minutes, using the filtering bag technique. The experimental design was entirely randomized in a 3x3 factorial scheme, with three different temperatures and autoclave execution time and three different roughage foods, with five replicates each. The results were submitted to analysis of variance and the averages compared using the Tukey test at 5% probability, using the statistical program R 3.6.1. Statistical difference (P<0,05) was observed between the different execution times and temperatures for the three roughage foods, because as the autoclave execution temperature increased, the lower was the value obtained for the acid detergent fiber. It was concluded that the use of different times and temperatures of autoclave execution using TNT filter bags (100g/m2) generates different results of acid detergent fiber in roughage foods.