Caracterização parcial de holocelulases e pectinases de isolado fúngico anemofílico cultivado em resíduos agrícolas

Abstract

Agricultural waste are produced in large amount every year in our country, and not always they have an appropriate purpose. Knowing that fungi naturally possess the ability to develop enzymes that can degrade the compounds of these residues, researchers have been trying to develop and optimize techniques to generate products of value from these residues. This project aimed to search in the Municipal "Parque do Mocambo an anemophilic fungus with the capacity to produce pectinases, cellulases and holocellulases for a possible application. The isolated fungus was distinquished in the production of xvlanases pectinases and CM-cellulases, mainly when cultivated in rice hull, whose values corresponded to 0,397 + 0,016, 0.230 + 0.054; and 0 197 +0.024 (Umt 1+ standard error), respectively partial enzymatic characterization tests. Evaluating the effect of temperature and pH, revealed the best profiles at 60 °C and pH 4.0 The application of enzymes from the crude extracts of rice hulls in hydrolysis tests of sugarcane bagasse samples showed a better response in a pre treated material with a steam explosion with a conversion peak of 10.54% of lignocellulosic material in reducing sugar.