Avaliação de diferentes métodos de conservação para transporte de testículos de cães destinados à coleta de sêmen do epidídimo

Abstract

The fauna is increasingly threatened with extinction by many factors, this fact makes necessary studies of techniques of conservation of the genetic material that enable the recovery and maintenance of endangered species, however, few studies report effective methods for the conservation of this material by long periods without significant loss of viability, especially when there is a need for emergency collection of samples from dead animals. The objective of this study was to evaluate different methods of preservation of the testis-epididymis complex for the collection of epididymal semen from dogs, in order to identify which method offers better conservation of the seminal sample. The testicles were collected after orchiectomy of 22 healthy adult dogs with no defined restrictions to breed or weight. The testes were randomly allocated into three groups, where each group was assigned to a different type of transport and the groups subdivided according to the transport time. Macroscopic volume, color and microscopic evaluations were performed, including motility, vigor, concentration and spermatic pathologies. Results obtained from right testicles immediately after orchiectomy were considered control; compared to those obtained from the left testicles from the same animal, in order to observe and compare the differences that occurred during the analyzes at different time intervals. After the analysis, it was possible to observe that the Styrofoam box proved to be more efficient than the thermal bag for the preservation of the testes-epididymis complex, with a lower loss in the seminal parameters. collection of semen from the epididymis.