Atividade antiproliferativa dos extratos hidrometanólico e acetato de etila de Sabicea brasiliensis em modelos celulares de câncer de mama

Abstract

Breast cancer is one of the most feared neoplasms by women. The diagnosis is commonly faced with difficulty and chemotherapy still causes side effects. The research on natural products as antineoplastic drugs is promising, allowing the identification of antitumor compounds that can control the proliferation of transformed cells. Sabicea brasiliensis is a plant usually found in Brazilian savanna, and exhibits medical properties such as anti-inflammatory, antioxidant and antinociceptive activities. The objective of this study was to evaluate the antiproliferative effect of the hydromethanolic and the ethyl acetate extracts from Sabicea brasilienses on the mammary lineages MCF-7 (tumorigenic with luminal phenotype) and MDA-MB231 (tumorigenic with triple negative phenotype) through MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazoliumbromide) assay. All cells were properly cultivated until reached 80% confluence. For the cell viability assay the extracts were used in the following concentrations 1ug/uL, 0,5ug/uL, 0,25ug/uL e 0,125ug/uL, 0,0625ug/uL, 0,03125ug/uL, 0,015625ug/uL e 0,0078125ug/uL and the cells treated for 24h and 48h. The hydromethanolic extract, in lug/uL concentration, presented cytotoxic effect to the MCF-7 cell, when compared to the control treated with diluent and to the MDA-MB231 lineage. The ethyl acetate extract demonstrated a peculiar behavior, inhibiting the proliferation of the luminal lineage upon 24 hours of treatment in its lower concentration. After 48 hours, this behavior was verified only for the treatment with lug/uL. Despite its intriguing behavior, the majority compounds need to be evaluated, as well as their mechanisms of action in the mammary tumor cells.