Avaliação da atividade do dióxido de cloro na cicatrização de feridas excisionais cutâneas em camundongos

Abstract

Due to the importance of cutaneous wounds several studies have been looking for compounds that avoid the high costs, the long periods of manufacture and the increase of bacterial resistance. In this way the chlorine dioxide (ClO2) is distinguished by the low cost, great biocidal capacity and its biocompatibility potential, besides presenting low toxicity when compared to other antiseptics like chlorhexidine. This work was carried out in two distinct stages, in which the concentration of chlorine dioxide to be used was determined in the first study by the technique of sponge implantation in the subcutaneous of mice to evaluate the dose-response curve, and in the second This concentration was tested in an experimental model of excisional skin wounds in mice. Soluble collagen, hemoglobin, myeloperoxidase activity (MPO) and N-acetyl-β-D-glicosaminidase (NAG) were measured in both steps and quantified total collagen deposition and types I and III. Based on the results presented by the technique of sponge implantation and taking into account the importance of angiogenesis and the presence of neutrophils and macrophages in the healing process, it was decided to test the concentration of 150 ppm of chlorine dioxide in the cutaneous healing process of mice. At days 1, 3, 7 and 14 days of treatment, the closure in% wound area of the group treated with chlorine dioxide at 150 ppm was higher when compared to the control group, with statistical difference at all times analyzed (p≤0,05 * ep≤0,01 **). The 7% stabilized chlorine dioxide solution at 150 ppm concentration was effective in accelerating the closure of excisional cutaneous wounds in mice, presenting a positive influence on tissue repair stages, from the inflammatory phase of the wound to the proliferative phase, with higher deposition of total collagen and types I and III at 14 days after the wound.