Estudo químico dos extratos das folhas de Cassia bakeriana biomonitorado pela atividade antifúngica

Abstract

Cassia bakeriana is a typical Thai tree, belonging to the Fabaceae family, popularly known as pink cassia. This species does not have many studies regarding the biological activity in relation to its extracts, but it belongs to a genus (Cassia) that has many published works with many evaluated biological activities. Thus, in this work the fractionation of the ethanolic extract from the leaves of C. bakeriana was performed through liquid-liquid extraction with solvents of different polarities, followed by phytochemical prospection in Thin Layer Chromatography (TLC) with specific developers for the main classes of secundary metabolites; the activity of the extracts and partitions against three species of fungi (that cause candidiases) was tested through the Minimum Inhibitory Concentration (MIC), where the dichloromethane partition (PD) was the most active for three species, being more active against the Candida glabrata, with an MIC value of 93,75 μg mL–1. Then, PD was the identification with Mass Spectrometry identified the main compounds as flavonoids such as kampferol (m/z 285.0407), (epi)-catechin (m/z 289.0715), flavonoids with glycoside groups as quercetin-3-O-glucoside (m/z 463.0880), phenolic acids such as p-coumaric acid (m/z 163.0402) and ferulic acid (m/z 193.0505), anthraquinone (m/z 283.0252) and some fatty acids. Based on studies found in the literature it is believed that these compounds may be related to the antifungal activity presented by the partition.