Efeito do glifosato sobre a estabilidade de eritrócitos humanos e de Rattus norvegiccus em solução salina fisiológica

Abstract

The bioaccumulation of pesticides can affect living organisms health through several different mechanisms, such as membranes denaturation. The evaluation of the deleterious effects of chemical agents on biological membranes can be done by analysis of the erythrocytes stability against a concentration gradient of certain chemical agent in physiologic saline solution. This work analyzed the effect of the herbicide glyphosate on hemolysis of human and rats bloods collected in EDTA or heparin as an anti-coagulant. The results were analyzed by spectrophotometry at 540 nm and light microscopy. There was an agreement between the spectrophometric and the morphologic analyzes. At the concentration range recommended for agricultural purposes, glyphosate promoted 100% of hemolysis both in rats and in humans. The glyphosate concentration capable of promoting 50% of hemolysis (D50) was significantly lower in rats than in humans. The D50 values of glyphosate obtained for the samples of human blood collected in EDTA were not significantly different than those obtained for the samples collected in heparin. However, the lysis curves presented lower values of absorbance at 540 nm in the presence of blood collected in EDTA in relation to that collected in heparin, probably due hemoglobin precipitation by EDTA.