Autotransplantação de ovário no subcutâneo e consumo folicular em gatas domésticas (felis catus)

Abstract

The main goal of this study was to establish the follicular depletion of the remaining and implanted ovaries in the subcutaneous in domestics cats after 120 days, by quantification and morphometric classification, and with respect to the preservation of the tissue organization. Thirteen female domestic cats (Felis catus) were used, distributed into two groups: G1 (cats 1 to 7, with left ovary as control and implant, and right ovary remaining), and G2 (cats 8 to 13, with right ovary as control and implant, and left ovary remaining). After unilateral ovariectomy, the ovary was sliced in three parts. The two extremities were implanted under the skin, and the middle section was used as control. Four months later, the implants and the remaining ovary were removed and observed under light microscopy. The implants were found in 38.4% of the cats, and the analysis showed a large number of primordial follicles (59.75%), primary follicles (40.25%), and conjunctive fibroplasia. The average size of the primary follicles was larger in the implanted ovary (36.10/mm²) than in the control ovary (12.11/mm²), and larger than in the remaining ovary (16.27/mm²). The remaining ovary showed regular appearance with follicles in all stages of development, with the average of the tertiary follicles (1.12/mm²) larger than the control (0.96/mm²). The diameter of primordial follicles was bigger in the control ovary (41,51μm) in relation to the remaining (37,30μm). The primary in the control was bigger (57,5 μm) than the remaining (47,5μm) and bigger than the implants (38,33μm). Considering the side, the tertiary follicles in the remaining had been more frequent in the right side and in relation to the diameter; the primary follicles in the controls had been bigger in the left side. We conclude that the ovarian implants recovered in this study only presented primordial and primary follicles, with fibroplasia. A length of study longer than 120 days to analyze follicular depletion would also be useful, because in that period this fact was not characterized.