Transporte e cultivo de embriões bovinos por 24, 48 e 72 horas antes da transferência

Abstract

It was evaluated the feasibility of the transport and cultivation in portable incubator of embryos produced in vitro by 24, 48 and 72 hours by assessing blastocyst rates and conception of the respective embryos. In vitro production of embryos obtained blastocyst rate of 18.19%, 22.28% and 22.72% in transport-cultivation for 24, 48 and 72 hours, respectively, with statistical difference for transport-cultivation by 24 hours. The results of conception rate after 30 and 60 days was similar on all days of cultivation and transportation of embryos, yielding rate 44.51%, 42.82% and 44.66% at 30 days post ET in 24, 48 and 72 hours of transportation and cultivation, respectively, and 41.12%, 40.99% and 42.42% at 60 days post ET in 24, 48 and 72 hours of transportation and cultivation, respectively. The transport and cultivation of embryos for 24, 48 or 72 hours before the transfer in portable incubators, were viable, regarding the conception rate, however the rate of blastocyst production was lower when transport and cultivation occurred for 24 hours.