Investigação in vitro dos efeitos do látex de jatropha multifida em fibroblasto humano HFF-1 e a modulação desses efeitos via síntese verde de nanopartículas de prata

Abstract

Skin diseases represent a significant public health problem, with particular concern for fibrosis, chronic wounds, or wounds that are difficult to heal. Given the limitations of current treatments, the search for new therapeutic alternatives has intensified, especially those involving natural compounds. The species Jatropha multifida has traditional use in the treatment of skin lesions, making it a potential source of bioactive compounds. Thus, this study aimed to evaluate the effects of the crude extract of the plant’s latex and of silver nanoparticles (AgNPs) synthesized via a green method on human fibroblast cells. The results showed that both the extract and the AgNPs interfere with cell viability, with up to an 80% reduction observed at extract concentrations above 375 µg/mL and at AgNP concentrations above 187.50 µg/mL. Furthermore, the evaluation of cell migration at three concentrations of the extract and AgNPs (23.44; 46.88; and 93.75 µg/mL) revealed an anti-migratory effect at all concentrations of the extract, while for AgNPs, this effect was observed only at concentrations of 46.88 and 93.75 µg/mL. Therefore, the data indicate that both the extract and the nanomaterial possess relevant biological activity, contributing to studies focused on cellular regeneration—either by controlling regeneration through anti-migratory properties or by replacing the extract with nanoparticles at less harmful concentrations.