Análise de diferentes protocolos de fibrina rica em plaquetas para a utilização em tratamento endodôntico de dentes com rizogênese incompleta: estudo in vitro

Abstract

Introduction: The treatment of necrotic teeth with incomplete root development has always been challenging due to the interruption of root formation. Regenerative endodontic therapy (RET) has emerged as an alternative approach. In this context, platelet-rich fibrin (PRF) stands out as an effective biological scaffold due to its potential to enhance tissue repair. Materials and Methods: S-PRF and PRO-PRF were obtained from venous blood using two different centrifugation protocols. Growth factor release was measured over a period of 10 days (240 hours), quantifying the release of EGF, TGF-β, PDGF, and VEGF at 6, 24, 48, 72, and 240 hours. The generated membranes were evaluated by histomorphometric analysis, considering fibrin network, platelet aggregates, and cellular morphology under light microscopy. Data were subjected to statistical analysis with a significance level of P ≤ 0.05. Results: Both protocols demonstrated similar behavior, with high growth factor release during the initial 6-hour period, followed by a reduction at 72 hours and a subsequent increase at 240 hours. A statistically significant difference was observed (P < 0.05), with S-PRF releasing a greater amount of growth factors. In contrast, no significant differences were found between groups regarding VEGF release (P > 0.05). Conclusion: Both protocols demonstrated potential for use in regenerative endodontic therapy by promoting growth factor release at all evaluated periods. S-PRF showed greater total release, whereas PRO-PRF demonstrated a more sustained release profile over time. Additional studies are needed to clarify the impact of these differences on tissue repair and regeneration.