EFICÁCIA DE Bacillus velezensis mandacaium, ISOLADO DO ALIMENTO LARVAL DE ABELHAS SEM FERRÃO, NO CONTROLE DA PÚSTULA BACTERIANA (Xanthomonas axonopodis pv. glycines) NA CULTURA DA SOJA

Abstract

The use of biological agents for plant disease control is a sustainable alternative as it reduces the reliance on chemical agents and mitigates soil and water contamination. In this study, various bacteria isolated from the larval food of stingless bees were evaluated for their antimicrobial action against Xanthomonas axonopodis. Bacillus velezensis demonstrated a high ability to produce compounds that inhibit the growth of X. axonopodis, with no toxicity observed when applied to soybean seeds. Moreover, complete genome sequencing revealed a genome size of 4,011 M bp and a GC content of 46,31 %. The analysis identified 4094 protein-coding genes, including at least 11 gene clusters with known antimicrobial properties. Thirteen genomic regions were predicted to contain putative gene clusters involved in metabolite biosynthesis. Additionally, metabolomic analysis identified 23 compounds, 8 previously unknown, with potential antimicrobial activity. These findings highlight the importance of Bacillus velezensis metabolites in controlling Xanthomonas axonopodis pv. glycines and their potential application in managing other plant diseases.