1. Repositório Institucional - Universidade Federal de Uberlândia
2. Instituto de Ciências Biomédicas (ICBIM)
3. TCC - Ciências Biomédicas

ORCID:	http://orcid.org/0009-0001-2822-8862
Document type:	Trabalho de Conclusão de Curso
Access type:	Acesso Aberto
Title:	Lectina de ligação à manose inibe a infecção pelo vírus Chikungunya
Alternate title (s):	Mannose-binding lectin impairs Chikungunya virus infection
Author:	Freitas, Lara Cristina Ferreira
First Advisor:	Jardim, Ana Carolina Gomes
First coorientator:	Santos, Igor Andrade dos
First member of the Committee:	Garcia Júnior, Marcelo Augusto
Second member of the Committee:	Silva, Poliana Gomes da
Summary:	Chikungunya virus (CHIKV), the etiological agent of chikungunya fever disease, is mainly transmitted by Aedes aegypty and A. albopictus mosquitoes. Symptoms of the disease include fever, join pain, as well as arthralgia and polyarthalgia, which can progress to a chronic condition for months or years. There are still no specific antivirals approved to treat CHIKV infection and, therefore, it currently threats public health systems. In this context, lectins isolated from plants have demonstrated diverse biological activities, providing an interesting source of molecules with antiviral activity, due to their ability to bind to specific sugar regions on viral particles, however, there is a lack of data for the understanding of the effects of lectins against CHIKV replication. Here the anti-CHIKV activity of four lectins isolated from different plants from the northeastern region of Brazil (ConA; PPL; MaL; and VML) was evaluated. For this, all lectins were screened towards their cytotoxicity in Syrian golden hamster kidney cells (BHK-21) at concentrations of 50, 10 and 2 µg/mL using cell viability assay after 16h. The highest non-cytotoxic concentration of each lectin was selected to evaluated their effect on CHIKV replicative cycle, employing a CHIKV infectious clone carrying the nanoluciferase gene (CHIKV-nanoluc). All lectins were tolerated by cells at 50µg/mL, but only ConA was active against CHIKV-nanoluc inhibiting virus replication by 96%. The ConA inhibition profile and cytotoxicity were further evaluated performing a dose-response assay in which resulted in a selectivity index of 14.71. Therefore, our data demonstrate that ConA has a potent antiviral activity against CHIKV. Further experiments are needed to be performed to investigate the mechanism of action of ConA, and also for a better understanding of its interactions with viral proteins. Finally, the data presented here can be used as a basis for future research that seeks to develop antiviral drugs against CHIKV.
Keywords:	Antiviral Chikungunya Vírus Lectins Natural compound
Area (s) of CNPq:	CNPQ::CIENCIAS BIOLOGICAS
Language:	eng
Country:	Brasil
Publisher:	Universidade Federal de Uberlândia
Quote:	FREITAS, Lara Cristina Ferreira. Lectina de ligação à manose inibe a infecção pelo vírus Chikungunya. 2024. 22 f. Trabalho de Conclusão de Curso (Graduação em Ciências Biomédicas) – Universidade Federal de Uberlândia, Uberlândia, 2024.
URI:	https://repositorio.ufu.br/handle/123456789/41972
Date of defense:	2-May-2024
Appears in Collections:	TCC - Ciências Biomédicas

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