Publicação:
Lectina de ligação à manose inibe a infecção pelo vírus Chikungunya

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dc.contributor.advisor-co1Santos, Igor Andrade dos
dc.contributor.advisor-co1Latteshttp://lattes.cnpq.br/6589573434199212pt_BR
dc.contributor.advisor1Jardim, Ana Carolina Gomes
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/7960921685798643pt_BR
dc.contributor.referee1Garcia Júnior, Marcelo Augusto
dc.contributor.referee1Latteshttp://lattes.cnpq.br/6720387318774380pt_BR
dc.contributor.referee2Silva, Poliana Gomes da
dc.contributor.referee2Latteshttp://lattes.cnpq.br/0364417521027402pt_BR
dc.creatorFreitas, Lara Cristina Ferreira
dc.creator.Latteshttp://lattes.cnpq.br/0247252287854480pt_BR
dc.date.accessioned2024-07-31T17:39:10Z
dc.date.available2024-07-31T17:39:10Z
dc.date.issued2024-05-02
dc.description.degreenameTrabalho de Conclusão de Curso (Graduação)pt_BR
dc.description.resumoChikungunya virus (CHIKV), the etiological agent of chikungunya fever disease, is mainly transmitted by Aedes aegypty and A. albopictus mosquitoes. Symptoms of the disease include fever, join pain, as well as arthralgia and polyarthalgia, which can progress to a chronic condition for months or years. There are still no specific antivirals approved to treat CHIKV infection and, therefore, it currently threats public health systems. In this context, lectins isolated from plants have demonstrated diverse biological activities, providing an interesting source of molecules with antiviral activity, due to their ability to bind to specific sugar regions on viral particles, however, there is a lack of data for the understanding of the effects of lectins against CHIKV replication. Here the anti-CHIKV activity of four lectins isolated from different plants from the northeastern region of Brazil (ConA; PPL; MaL; and VML) was evaluated. For this, all lectins were screened towards their cytotoxicity in Syrian golden hamster kidney cells (BHK-21) at concentrations of 50, 10 and 2 µg/mL using cell viability assay after 16h. The highest non-cytotoxic concentration of each lectin was selected to evaluated their effect on CHIKV replicative cycle, employing a CHIKV infectious clone carrying the nanoluciferase gene (CHIKV-nanoluc). All lectins were tolerated by cells at 50µg/mL, but only ConA was active against CHIKV-nanoluc inhibiting virus replication by 96%. The ConA inhibition profile and cytotoxicity were further evaluated performing a dose-response assay in which resulted in a selectivity index of 14.71. Therefore, our data demonstrate that ConA has a potent antiviral activity against CHIKV. Further experiments are needed to be performed to investigate the mechanism of action of ConA, and also for a better understanding of its interactions with viral proteins. Finally, the data presented here can be used as a basis for future research that seeks to develop antiviral drugs against CHIKV.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo a Pesquisa do Estado de Minas Geraispt_BR
dc.identifier.citationFREITAS, Lara Cristina Ferreira. Lectina de ligação à manose inibe a infecção pelo vírus Chikungunya. 2024. 22 f. Trabalho de Conclusão de Curso (Graduação em Ciências Biomédicas) – Universidade Federal de Uberlândia, Uberlândia, 2024.pt_BR
dc.identifier.urihttps://repositorio.ufu.br/handle/123456789/41972
dc.languageengpt_BR
dc.orcid.putcode164717406
dc.publisherUniversidade Federal de Uberlândiapt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.courseBiomedicinapt_BR
dc.rightsAcesso Abertopt_BR
dc.sizeorduration22pt_BR
dc.subjectAntiviralpt_BR
dc.subjectChikungunya Víruspt_BR
dc.subjectLectinspt_BR
dc.subjectNatural compoundpt_BR
dc.subject.cnpqCNPQ::CIENCIAS BIOLOGICASpt_BR
dc.titleLectina de ligação à manose inibe a infecção pelo vírus Chikungunyapt_BR
dc.title.alternativeMannose-binding lectin impairs Chikungunya virus infectionpt_BR
dc.typeTrabalho de Conclusão de Cursopt_BR
dspace.entity.typePublication

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