Publicação: Lectina de ligação à manose inibe a infecção pelo vírus Chikungunya
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CNPQ::CIENCIAS BIOLOGICAS
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Santos, Igor Andrade dos
Resumo
Chikungunya virus (CHIKV), the etiological agent of chikungunya fever disease, is
mainly transmitted by Aedes aegypty and A. albopictus mosquitoes. Symptoms of the disease
include fever, join pain, as well as arthralgia and polyarthalgia, which can progress to a chronic
condition for months or years. There are still no specific antivirals approved to treat CHIKV
infection and, therefore, it currently threats public health systems. In this context, lectins
isolated from plants have demonstrated diverse biological activities, providing an interesting
source of molecules with antiviral activity, due to their ability to bind to specific sugar regions
on viral particles, however, there is a lack of data for the understanding of the effects of lectins
against CHIKV replication. Here the anti-CHIKV activity of four lectins isolated from different
plants from the northeastern region of Brazil (ConA; PPL; MaL; and VML) was evaluated. For
this, all lectins were screened towards their cytotoxicity in Syrian golden hamster kidney cells
(BHK-21) at concentrations of 50, 10 and 2 µg/mL using cell viability assay after 16h. The
highest non-cytotoxic concentration of each lectin was selected to evaluated their effect on
CHIKV replicative cycle, employing a CHIKV infectious clone carrying the nanoluciferase
gene (CHIKV-nanoluc). All lectins were tolerated by cells at 50µg/mL, but only ConA was
active against CHIKV-nanoluc inhibiting virus replication by 96%. The ConA inhibition profile
and cytotoxicity were further evaluated performing a dose-response assay in which resulted in
a selectivity index of 14.71. Therefore, our data demonstrate that ConA has a potent antiviral
activity against CHIKV. Further experiments are needed to be performed to investigate the
mechanism of action of ConA, and also for a better understanding of its interactions with viral
proteins. Finally, the data presented here can be used as a basis for future research that seeks to
develop antiviral drugs against CHIKV.
Abstract
Palavras-chave
Antiviral, Chikungunya Vírus, Lectins, Natural compound
Citação
FREITAS, Lara Cristina Ferreira. Lectina de ligação à manose inibe a infecção pelo vírus Chikungunya. 2024. 22 f. Trabalho de Conclusão de Curso (Graduação em Ciências Biomédicas) – Universidade Federal de Uberlândia, Uberlândia, 2024.