Efeito neuroprotetor do resveratrol (3,4′,5-trihidroxi-estilbeno) sobre comportamentos do tipo depressivos em filhotes fêmeas de mães tratadas com antígenos suspensos na vacina influenza tetravalente

Abstract

Among mental disorders, depression represents a frequent disorder and an important public health problem. Considered a multifactorial disease, its etiology may be associated with genetic, psychosocial and biological factors. Given the complexity of the processes that occur during pregnancy, environmental disturbances during this period can activate the maternal immune system and cause neuropsychiatric changes in the offspring. Among natural compounds, resveratrol stands out as a promising adjunct to the classic pharmacological treatment of diseases such as depression, due to its antioxidant and anti-inflammatory properties. Therefore, this study aimed to investigate whether the induction of maternal immune activation caused by antigens suspended in the tetravalent influenza vaccine causes depressive-like behavioral changes in the offspring of female mice of the Balb/C lineage. Furthermore, this research also sought to evaluate the neuroprotective effect of resveratrol on such behavioral impairments through the Forced Swim and Sucrose Consumption tests. For this, the animals were divided into four groups, as follows: Methyl-PBS (pups from mothers who received 0.5% carboxymethylcellulose, from E0 to E21 + 1 intramuscular injection of PBS at E16); Methyl-VAC (pups from mothers who received 0.5% carboxymethylcellulose, from E0 to E21 + 1 intramuscular injection of tetravalent influenza vaccine at E16); RESV-PBS (pups from mothers that received resveratrol, from E0 to E21 + 1 intramuscular injection of PBS at E16); RESV-VAC (pups from mothers who received resveratrol, from E0 to E21 + 1 intramuscular injection of tetravalent influenza vaccine at E16). The results presented in this study demonstrated that there was no significant difference between the groups of animals in relation to the immobility time in the NF test. Regarding the CS test, an increase in sucrose preference was observed in the Methyl-VAC and RESV-VAC groups. Furthermore, there was also a significant increase in preference for the sucrose solution by the VCC group compared to the RESV group. There was also a significant increase in preference for the sucrose solution by the Methyl-VAC group in relation to the RESV-PBS group. Given this, we can conclude that exposure to antigens suspended in the tetravalent influenza vaccine did not cause depressive-type behavioral changes in the offspring of adult females, just as resveratrol did not demonstrate a neuroprotective effect under the conditions of this experiment.