Emprego do kit portátil DOT-ELISA para detecção de anticorpos IgG de Neospora caninum no soro de bovinos leiteiros, Uberlândia- MG

Abstract

Since neosporosis is of paramount importance for livestock, not only in Brazil, but worldwide, several studies were carried out in order to verify the occurrence and prevalence of the disease in the herd. It is an infectious disease that has been highlighted as an important cause of reproductive problems, where there is an increase in the occurrence of abortions and neonatal mortality. Caused by an obligate intracellular protozoan, which affects not only cattle, but other species as well. Its main definitive host is canids, especially the domestic dog, which lives in a rural environment. In this work, we used the ImmunoComb portable DOT-ELISA kit to detect IgG antibodies in bovine serum and verify the occurrence of the protozoan Neospora caninum in dairy cows at the Experimental farm of the Federal University of Uberlândia. The kit had 30 tests to be used individually. As a result, 23.4% (7/30) of the females tested had Neospora caninum IgG antibodies. With the use of this test and in case it was necessary to verify the herd's reproductive notes, we would have control or even explanations for possible occurrences of abortion, repetitions of estrus and / or early estrus entry by an animal, which happened in a period prior to the performance of the test. test. With these data, we would have a guide in which managements would be necessary to make changes on the farm, in order to avoid loss of calves, or else the permanence of infected animals, as this would lead to mass contamination of the herd, since the clinical signs presented by animals make us relate to other reproductive diseases and this would lead to a drop in production and a significant increase in breeding costs, especially those related to animal health.