Análise da própolis verde brasileira na proliferação e viabilidade de células pulpares humanas

Abstract

The innovations of biomaterials for tissue repair in dentistry are associated with advances in therapeutic approaches aimed at maximum preservation of healthy structures, called Minimally Invasive Dentistry. At the same time, phytotherapy is gaining ground and biomaterials of natural origin have greater chances of biocompatibility with the host. In this context, the present study aimed to analyze the direct effect of Brazilian green propolis (BGP) on human pulp cells, in order to assess the potential for cell viability and tissue repair. Thus, the pulp cells were seeded in 96-well plates (10,000/well) and after 24h they were submitted to the direct application of the materials: PVB in different concentrations (5, 10 and 50 μg/mL), 0.5% DMSO (control of propolis diluent), carbamide peroxide (PC - negative control group) 0.018% and DMEM (positive control). Cell viability was evaluated by the MTT test, 24 hours after application. In addition, the cell migration test (wound healing) compared immediate contact and after 24h and 48h after an injury in the environment. Data analysis used the One-Way ANOVA test complemented by Tukey (α= 5%). Regarding cell viability and migration results, there was no statistical difference between the experimental group (PVB) and the positive control (p<0.05). Unlike the negative control group (PC), which presented intercellular spaces, caused by the decrease in the number of cells, alterations in the format of the original membrane and irregular cell formats. It was concluded that PVB showed low cytotoxicity, high cell tolerance and ability to induce cell proliferation in pulp tissues.