Pesquisa de Malassezia pachydermatis em conduto auditivo de cães com comorbidades sem queixa de otite

Abstract

It is known that Malassezia pachydermatis is a fungus naturally found in the skin flora of dogs and cats. Its presence does not interfere with the animal's life and does not interfere with its well-being. However, Malassezia sp. it has an opportunistic behavior and, therefore, can cause harm to those animals that are immunosuppressed or have comorbidities. Therefore, when the dog or cat is vulnerable to another disease, Malassezia sp. proliferates causing an imbalance in the dermis and resulting in dermatitis. The dermatopathies frequently associated with malasseziosis are: atopic dermatitis, food allergy, dyskeratosis, endocrinopathies, prolonged treatment with corticosteroids and antibiotics, in addition to otopathies. Malasseziosis causes changes in the skin surface, causing the disruption of the epidermal barrier. The objective of this study was to evaluate the presence of Malassezia pachydermatis and its quantity in the auditory canal of dogs with comorbidities. In addition, a survey was made of which breeds and diseases were associated with the proliferation of this yeast. To carry out the experiment, swabs were used to collect cerumen from the ear canals of dogs. In all, the experiment included the analysis of cerumen from 50 animals, 10 of which belonged to the negative control group. In the control group there was no disease. The dogs used in this category were all healthy and had not been treated with antifungals for at least the last 6 months. The results obtained from the sick dogs were compared with those from the control group. The collected material was transferred to slides, stained with fast panoptic. The slides were evaluated via microscope, in 40 fields, at 40 and 100 times magnification, with the last addition of immersion oil. Of the total of 40 sick dogs analyzed, 45% presented positive results for Malassezia pachydermatis and 55% negative, unilaterally or bilaterally. Of the 10 animals analyzed in the control group, 30% were positive for M. pachydermartis, and the other 70% were negative. The experiment concluded that the Malassezia pachydermatis yeast may be present in sick dogs subclinically.