Avaliação de impressos de alginato e gelatina utilizando a bioimpressora Allevi 2

Abstract

3D bioprinting is based on the principles of conventional 3D printing, but can be used to build components that mimic tissues thanks to the insertion of cells and growth factors. This makes it possible to reproduce its physiology in vitro and, eventually, implant imprints in vivo, enabling several applications in regenerative medicine. There are several 3D bioprinting techniques and types of materials, especially hydrogels. The objective of this work was to evaluate the printability of an alginate and gelatin hydrogel, so initial printing tests were carried out with an acrylate-based gel, followed by printing and analysis of the alginate and gelatin blend, in the form of cylinders, parallelepipeds and prisms in triplicate, using the Allevi 2 3D bioprinter. There was great variation in the X, Y, Z axes, base areas, perimeters and roundness between the samples, justified by the rheological characteristics of the materials used. In addition, it was found that parameters such as pressure and temperature may represent future challenges in the use of cells. 3D bioprinting should be further studied, considering the importance of its contribution to regenerative medicine.