Avaliação do potencial antioxidante e antiadipogênico do extrato acetato de etila e proantocianidinas do araticum (Annona crassiflora Mart.) em adipócitos 3T3-L1

Abstract

Obesity is a worldwide public health concern, as its exponential growth illustrates its pandemic characteristic. This metabolic disorder results from an increase in the size and number of adipocytes and may favor the development of metabolic disorders such as type 2 diabetes mellitus, cardiovascular disease and osteoporosis. In obesity, there is a close relationship between excessive weight and oxidative stress, in which the levels of reactive oxygen species (ROS) increase significantly. Studies point out that oxidative stress contributes to the intensification of adipogenesis by increasing the activation and expression of adipogenic proteins. In this sense, the application of antioxidant compounds in obesity therapy is interesting for an anti-obesity effect. It is known that the ethyl acetate and proanthocyanidin fractions of the peel of the araticum fruit (Annona crassiflora Mart.) have antioxidant activity. Thus, the objective of this work is to evaluate the effects of araticum peel extract fractions on 3T3-L1 cells and the possible anti-adipogenic activity through the study of the antioxidant capacity of treatments and methods of coloring intracellular lipids (Oil Red O and Nile Red). The treatments were performed at different concentrations (200; 100; 50; 25; 12.5; 6.25; 3.125 and 1.5625 µg/mL), however, it was not possible to confirm whether they presented adipogenesis inhibitory activity, since there was no adipogenic induction of 3T3-L1 preadipocytes. Furthermore, it is not possible to relate the production of ROS with adipogenesis. However, it is possible to infer that the ethyl acetate and proanthocyanidin fractions of araticum have antioxidant activity.