Microbioma do alimento larval de abelhas sem ferrão: diversidade e potencial biotenológico

Abstract

Given the importance of stingless bees as the main pollinators of native species in Brazil and the scarcity of data on the microbiota present in this group, this study will generate knowledge about the microbiota associated with this highly social insect. This research will focus on the biotechnological use of bacteria isolated from the larval food of stingless bees. In the first chapter of this research, we deal with the importance of stingless bees, the composition of larval food, and its importance for the differentiation of castes of these bees. As well as the importance of the microbiota that makes up this larval food. The potential for biotechnological use of the products of stingless bees and the microorganisms associated with these bees; The use of NGS and isolation tools for the characterization of microorganisms; And finally, the importance of discovering antimicrobials for use against multidrug-resistant bacteria. In Chapter 2, we present the article entitled: “Microbiota associated with larval food of Brazilian stingless bees: F. varia, M. quadrifasciata, M. scutellaris, and T. angustula”, available in submission format for publication. This research brings unprecedented results on the microbiota associated with stingless bees Frieseomelitta varia, Melipona quadrifasciata, Melipona scutellaris, and Tetragonisca angustula. Contributing to improving the understanding of the role of these microorganisms in the development of bees. In Chapter 3, we present the article “Antimicrobial activity of supernatants produced by bacteria isolated from Brazilian stingless bee’s larval food”; already been published. In this work, the antimicrobial effects of different bacterial supernatants isolated from the larval food of stingless bees of the species Melipona quadrifasciata, Melipona scutellaris, and Tetragonisca angustula are evaluated. Our investigations resulted in the discovery of bacteria with the potential to produce antimicrobial molecules.