Avaliação do uso de membrana de colágeno tipo II no processo de cicatrização de lesões experimentalmente induzidas em ratos Wistar

Abstract

Due to the growing market and interest in new sciences that aim to help or improve wound healing time, it is important that the surgeon is aware of the factors that lead to tissue recomposition or, at least, to epithelial continuity. The skin is one of the most important organs, as it protects the internal environment from the external one, maintaining homeostasis and regulating temperature. The aim of this study was to verify the action of type II collagen in the repair process of excisional skin wounds created experimentally in Wistar rats. A total of 54 male Wistar rats, weighing approximately 300 grams, were submitted to the creation of a 7.5mm excisional skin wound near the withers. Daily, after surgery, animals in the "positive control" and UCII-treated groups were submitted to wound cleaning using gauze soaked in ultrapure water. In the positive control group, after cleaning, 1 mL of ultrapure water was instilled over the wound. In the treatment group, immediately after cleaning, the wound was covered by a film of non-hydrolyzed bovine collagen that was hydrated in the wound. The samples were submitted to macroscopic analysis of total collagen where it was possible to verify that, in the treated group, there was an increase in the percentage of collagen in the wound and a significant decrease in crust, meaning that the healing rate was faster in the treated group.