Perfis de virulência de Salmonella Typhimurium isoladas de alimentos e amostras clínicas de humanos

Abstract

Foodborne diseases (DTA’s) are a major public health problem worldwide and some of the main pathogens involved are representatives of the Salmonella genus. The Typhimurium serovar is one of the most recurrent and affects its hosts causing enteric disorders such as vomiting, abdominal pain, fever and diarrhea. The objective of this study was to verify the presence of virulence genes in S. Typhimurium from 43 isolates from food and clinical samples of humans from the Biological Collection of Bacteria of Interest to Health of the Oswaldo Cruz Institute (IOC / FIOCRUZ). The sefA, lpfA and agfA, sivH, hilA, invA and sopE, avrA and spvC genes were searched using the conventional PCR (polymerase chain reaction) technique and the results were used to create virulence profiles. The general results of the samples show that the strains studied presented at least five virulence genes, which, if expressed, may represent a risk to public health. The positivity for the lfpA gene was 95.3% (41/43), for avrA 97.6% (42/43), for spvC 39% (17/43), for sopE 6.9% (03/43 ) and for sefA 0% (0/43). However, the diversity of locations and origins of the isolates did not allow an epidemiological relationship, requiring further studies using techniques that allow this type of correlation.