Proteínas da matriz do esmalte na formação de tecido mineralizado em áreas enxertadas com osso bovino desproteinizado

Abstract

This study evaluated the effect of the association of the enamel derivate matrix proteins (EMD) with deproteinized bovine bone (DBB) in the formation of mineralized tissues, through microtomographic analysis on rat jaws. In this study, 24 rats were divided into two groups according to the association of biomaterials used in the grafting procedure: DBB: Deproteinized bovine bone. EMD: Association of the DBB with EMD. The surgical procedure consisted of opening a flap and exposing the lateral face of the mandibles, which were later submitted to the adaptation of a dome-shape Teflon membrane. This membrane was filled with the DBB and EMD. The animals were euthanized in two experimental periods (30 and 90 days, n=6 animals), and their jaws were scanned in a micro-CT scanner. This methodology was applied to evaluate the volume of mineralized tissue that was found between the membrane and the mandible in percentage. It was verified that the DBB group presented a volume of mineralized tissue of 63.32 ± 7.15% at 30 days and 59.69 ± 11.24% at 90 days, while the EMD group presented a mineralized tissue volume of 60.14 ± 3.69% at 30 days and 67.62 ± 9.74 % at 90 days. There were no statistically significant differences between groups. EMD did not influence the formation of mineralized tissues in areas grafted with DBB.