Uso do selante de fibrina BthTL e do peptídeo mimético de TNF na lesão de nervo isquiático: impacto nas respostas na medula espinal

Abstract

Peripheral nerve injuries are recurrent, as they are widely distributed throughout the body. In cases of neurotmesis-type injuries (total section), surgical intervention is necessary to realign the stumps so that regeneration occurs linearly and without the occurrence of neuromas. The neuronal response to this type of injury will depend on factors such as the severity of the injury, neuronal type, proximity of the injury to the cell body and whether the interaction of the Central Nervous System with the target is restored, since the nerve cell bodies reside in the spinal cord and dorsal root ganglia. In view of this, our team demonstrated that the BthTL fibrin sealant associated with a peptide with a mimetic action to TNF resulted in a greater number of inflammatory cells and Schwann cells with pro-regenerative phenotype, which resulted in rapid remyelination and greater repair efficiency. Knowing that a nerve injury causes retrograde changes in the cell body and in surrounding cells, this work analyzed the changes at the spinal level. Neuron counts in the anterior horn and GFAP and Iba-1 expression analysis were performed to identify glial activity. Considering the results obtained, filling tubes with BthTL sealant, either alone or associated with the TNF-mimetic peptide, does not provide changes in the variables analyzed in the spinal cord after the treatment of sciatic nerve injury.