Hidroxichalconas com potencial antibacteriano e antibiofilme frente a bactérias periodontopatogênicas

Abstract

Objectives: Periodontitis is a pathology resulting from complex interaction between the microorganisms present in the biofilm and the host's immune response. Increased use of antibiotics, associated with their inappropriate use has increased the resistance of anaerobic bacteria. Therefore, identifying new antimicrobial substances, such as chalcones, is urgent. This study evaluates the antibacterial activity and the antibiofilm activity of 15 hydroxychalcones against periodontopathogenic bacteria. Methods: The compounds were evaluated by minimum inhibitory concentration (MIC) and minimum inhibitory concentration of biofilm (MICB) tests. Results: Samples T3, Li-6, Li-9, Li-40, Li-42, and LR-72 showed good antibacterial and antibiofilm activity for most of the evaluated bacteria: MIC was lower than or equal to 6.25 μg/mL, biofilm biomass was reduced by 95%, and cell viability was totally inhibited for sample concentrations between 0.78 and 100 μg/mL. Among the tested chalcones, Li-6 stood out: it was effective on all bacteria, as revealed by both MIC and MICB results. Conclusions: Our results have consolidated a base for the development of new studies on the effects of the tested molecules as agents to combat and to prevent periodontitis.