Determinação da curva de crescimento de Metapneumovírus aviário subtipo A em cultura de células primárias de fibroblasto de embrião de galinha

Abstract

The commercial production of chickens can be affected by several diseases, such as: Avian Coccidiosis, Avian Aspergillosis, Avian Clostridiosis, Avian Colibacillosis, Avian Salmonellosis, Avian Mycoplasmosis, Infectious Chicken Anemia, Infectious Bronchitis of Chickens, Marek's Disease, Gumboro Disease, Avian Encephalomyelitis, Avian Influenza and others. In this scenario, the Avian Metapneumovirus (aMPV) has acquired a prominent role among respiratory pathogens, triggering severe health problems, mainly because it is associated with secondary infections, causing significant economic losses. The aMPV has a negative sense, single-stranded RNA genome and it is enveloped, being the primary causative agent of Turkey Rhinotracheitis (TRT), and it is also constantly associated with Swollen Head Syndrome in chickens (SHS), affecting broilers, layers and breeders. Thus, it constitutes a significant source of economical losses for the process of commercial breeding of these animals. The main objective of the present work was to evaluate three different inoculated amounts of the aMPV vaccine strain in primary cells of chicken embryos, in order to determine which inoculated amount will be responsible for the highest viral yield in the culture. For this, we evaluated the growth curve in each condition, to verify the time point when the viral titer reach the peak in each condition, in order to establish the ideal time for virus harvesting and optimize the yield for the cultivation of the aMPV vaccine strain.