GM-CSF, IFN-γ, IL-3 e IL-6 em cistos radiculares humanos

Abstract

The major stimuli for the development of root cysts, associated with Malassez's epithelial remains, are bacterial endotoxins that act as mitogens and stimuli for the production of cytokines. The performance of certain cytokines released by the cells of the immune system has an important role in cystic growth and consequent destruction of the surrounding bone tissue. The objective of this work was to determine some cytokines related to the inflammatory process and bone resorption: GM-CSF, IFN-y, IL-3 and IL-6; in the liquid and tissue of human root cysts, using an enzyme immunoassay (ELISA). The detection limit of the tests by the ELISA ranged from 62.5 to 125pg / ml for GM-CSF; from 9.8 to 39, lpg / ml for IFN-y; 250 to 500pg / ml for IL-3 and 9.4 to 18.8pg / ml for IL-6. The results obtained in the cystic liquid samples were 42.8% of the lesions, with GM-CSF (66 to 1180pg / ml) and 92.8%, with IL-6 (47 to 4096pg / ml). IFN-y and IL-3 cytokines were not detected in any sample. The results obtained in the cystic tissue samples were 9.8% of the lesions reactive for IFN-y (53 to 71pg / ml), 28.6% for IL-3 (277 to 713pg / ml), 86.4% for EL-6 (17 to 835pg / ml) and 95.8% for GM-CSF (68 to 496pg / ml). With this study, it can be said that there is the occurrence of the cytokines IFN-y and IL-3 in the tissue of root cysts, despite being present in a low number of lesions and that GM-CSF and IL-6 are also present in the fluid and tissue of human root cysts. These cytokines may be participating in the inflammatory process and cystic growth, as well as in the characteristic bone resorption at the site of these lesions.