Avaliação de sinais eletrofisiológicos e desenvolvimento de imunossensores para detecção de troponina T e proteína C reativa

Abstract

The electrocardiogram (ECG) is one of the complementary exams with most informative capacity used for diagnosis, assessment of severity and treatment planning in acute coronary syndrome. In addition to the electrocardiographic changes, it is observed the importance of quantification of molecular markers of myocardial injury and inflammation markers. The detection the biomarkers may be performed in an optimized way from the development of biosensors, which have detection capability in real time with high specificity, selectivity and sensitivity. The main objective of this research is to evaluate quantitative measures of electrocardiographic signs performed in patients affected by myocardial infarction and in patients without changes related to acute coronary syndrome, and the development of immunosensors for detection of biomarkers related to myocardial infarction, troponin T (TnT) and C-reactive protein (CRP). The data were electrocardiogram and molecular markers of myocardial injury. Initially, it was developed a software to obtain digital electrocardiographic signals for further calculation and analysis of the entropy of electrocardiograms. Furthermore, from the development of immunosensors for detection and quantitation of CRP and cTnT, it becomes possible to evaluate molecular markers of myocardial injury. From obtaining electrophysiological signals and values of biomarkers from immunosensors, it is possible the diagnosis, evaluation and monitoring of patient outcomes. The results of this study can contribute to the improvement of diagnostic methods and better prognosis of acute myocardial infarction.