Influência do tempo de congelamento do soro sanguíneo nas concentrações séricas de minerais e enzimas em bovinos da raça Bonsmara

Abstract

The influence of storage time and temperature on the stability of blood biochemical 8 parameters is important on the diagnosis and prognosis of diseases and evaluation of the nutritional 9 status of domestic animals. The objective of this study was to evaluate the effect of storage time on 10 serum concentrations of minerals and enzymes in Bonsmara cattle. Blood samples were collected from 11 80 animals by jugular venipuncture into tubes without anticoagulant to obtain serum. The separate 12 serum were analysed on the day of collection, after 30 and 60 days at -20º C freezing temperature. The 13 concentrations of calcium (Ca+), phosphorus (Pi), Ca+: Pi ratio, magnesium (Mg), Iron (Fe), alkaline 14 phosphatase (FAL), gamma glutamyltransferase (GGT), creatine kinase (CK), alanine 15 aminotransferase (ALT) and aspartate aminotransferase (AST) were assayed with a Chemwell® 16 automated analyzer. The data were analyzed by Kruskal Wallis test with 5% confidence. Significant 17 reduction (p<0,05) were observed in Ca+, Pi, Mg, ALT, GGT values and Ca+: Pi ratio increased with 18 60 days stored at -20º C. The storage of the serum sample under freezing at -20º C for several days 19 causes a statistically reduction in the values of most of the analyzed elements.