Análise da taxa de produção in vitro de embriões bovinos com a utilização do resveratrol na maturação oocitária

Abstract

The in vitro embryo production (IVP) is a biotechnology that aims to increase the speed of production of genetically superior animals. This process is still considered inefficient and stagnant, presenting low embryo production and inferior quality. The objective of this study was to evaluate the effects of nanoparticles linked to resveratrol as antioxidants during the in vitro maturation of bovine oocytes on cleavage and blastocyst rates. The oocytes used for in vitro embryo production were obtained by the method of aspirations of ovarian follicles collected from cows in a slaughterhouse in the city of Uberlândia-MG, varying the breed, age and weight of the animals. After selection the oocytes proceeded to the in vitro maturation (IVM) stage, divided according to the treatments: control, resveratrol diluted at 0.5 µM and 1.0 µM and resveratrol bound to silica nanoparticles at concentrations 0.5 µM and 1.0 µM. Fertilization was performed on the IVF plate and with semen selected form the same bull for all replicas. After the IVG period, the possible zygotes were transferred to the CIV culture plate. Forty-eight hours after fertilization, the number of dividing structures (cleavage rate) was counted and after seven days, the number of blastocysts (blastocyst rate) was evaluated. The numbers were analyzed by logistic regression in the MINTAB program, and the treatment effect was included in the model. Statistical significance was set at P ≤ 0,05 and statistical trend at 0,05 < P ≤ 0,10. The use of 0.5 µM resveratrol and resveratrol bounded to nanoparticles at 0.5 µM and 1 µM did not show any advantage for embryo production rate then compared to the untreated group. However, the use of resveratrol at 1 µM concentration reduced the cleavage rate and tended to reduce the blastocyst rate. Also, the use of resveratrol at 1.0 µM concentration associated to nanoparticles appeared to be advantageous because it did not cause any detrimental effects on in vitro production.