Efeito protetor de antioxidantes (vitaminas C, E e beta-caroteno) e minerais (cobre, selênio e zinco) contra a ação genotóxica da doxorrubicina em células somáticas de Drosophila melanogaster

Abstract

Vitamins are important biological components that are able to deactivate highly active molecules such as, free radicais, generated throughout biochemical processes of the organism. Currently, chemotherapy used in the treatment of câncer, generates free radicais as a side effect of this type of therapy drug. An example of this is Doxorubicin (DXR). When used in association with chemotherapy and multivitamins, the wing spot test was administered to Drosophila melanogaster (somatic mutation and recombination test- SMART) in order to evaluate possíble genotoxic effect of a multivitamin compound (Vitamin C, E and beta- carotene) and minerais (copper, selenium and zinc), commercially known as Vitergan® Zinc Plus (12.5; 25 and 50mg/mL). The objective of this study was to evaluate the genotoxic effect of these multivitamins and minerais as well as their antigenotoxic effects as compared to the genotoxic effect of DXR (0.125mg/mL), producing free radicais. Results showed no statistically significant19 increase in the frequency of mutation spots, as comparecí to the negative control group with the multivitamin group, in the trans-heterozygous descendants of both crosses. However, there was a statistically significant reduction in the number of spots when the multivitamin is given in conjunction with DXR (0.125mg/mL) to trans-heterozygous descendants of both crosses. The protector effect was observed when the larvae received pre and co-treatments with the multivitamin in conjunction with DXR. Therefore, it is possible to conclude that in these experimental conditions, the multivitamin and minerais are not genotoxic. However it demonstrates a protective effect against the genotoxic effect of the chemotherapeutic agent DXR.