Efeito do selante de fibrina BthTL na regeneração axonal e recuperação funcional após lesão nervosa periférica

Abstract

In case of a peripheral nerve injury, the procedure has limitations regarding the type and time of injury, not having a positive result regarding the recovery of the function. The use of tubular prostheses that guide and protect the area of injury and the possibility of inserting substances that act positively into the nerve regeneration process within this prosthesis is a perspective in current practice. In this sense, this paper aimed to study the axonal growth and schwann cell responses in nerves damaged by total transection (neurotmesis), submitted to the tubulization process (the nerve stumps are inserted into a silicone tubular prosthesis) with the use of a fibrin sealant developed at our university (patent pi-0406273-6), which provides a biological framework within the prosthesis that may favor axonal recovery. 45 days after the injury, the nerves were analyzed and compared with control groups. For the study we used functional analysis (motor and sensory) and immunofluorescence. Regarding motor recovery, the present study observed an improvement over time, but did not obtain significant improvement comparing the groups. Immunofluorescences showed more presence of pro-regenerative phenotypes in the tube+sealant group. The perspective is that the work contributed with the repair technique and use of sealant to improve/assist the recovery of the peripheral nerve. In addition, further research with other substances that may have an effect on regeneration and that have greater efficiency in the functional aspect is encouraged, returning movements and sensitivity to the individual affected by the injury.